Table 1.

Chemicals, peptides and Recombinant Proteins

	Reagent or resource antibody	Source	Identifier
1	PE/Cy7 anti-mouse CD3	Biolegend	100220
2	APC anti-mouse CD4	Biolegend	100412
3	Alexa Fluor® 700 anti-mouse CD25	Biolegend	102024
4	PerCP/Cyanine5.5 anti-mouse CD8a	Biolegend	100733
5	Pacific Blue™ anti-mouse/human CD44	Biolegend	103020
6	Brilliant Violet 605™ anti-mouse CD45	Biolegend	103139
7	PE/Dazzle™ 594 anti-human/mouse Granzyme B Recombinant	Biolegend	372207
8	Alexa Fluor® 488 anti-mouse FOXP3	Biolegend	126405
9	PE anti-mouse H-2Dk	Biolegend	110307
10	Brilliant Violet 421™ anti-mouse F4/80	Biolegend	123131
11	PE/Dazzle™ 594 anti-mouse I-A/I-E	Biolegend	107647
12	FITC anti-mouse CD86	Biolegend	105005
13	PE/Cy7 anti-mouse/human CD11b	Biolegend	101215
14	APC anti-mouse CD11c	Biolegend	117309
15	PerCP/Cyanine5.5 anti-mouse CD49b (pan-NK cells)	Biolegend	108915
16	Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1)	Biolegend	108421
17	PE/Cy7 Rat IgG2b, κ Isotype Ctrl	Biolegend	400617
18	APC Rat IgG2b, κ Isotype Ctrl	Biolegend	400611
19	PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl	Biolegend	400531
20	Pacific Blue™ Rat IgG2b, κ Isotype Ctrl	Biolegend	400627
21	Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl	Biolegend	400657
22	Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl	Biolegend	400625
23	PE Mouse IgG2a, κ Isotype Ctrl	Biolegend	400211
24	Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl	Biolegend	400549
25	PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl	Biolegend	400659
26	FITC Rat IgG2a, κ Isotype Ctrl	Biolegend	400505
27	Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl	Biolegend	400628
28	TruStain fcX™ (anti-mouse CD16/32)	Biolegend	101320
29	True-Nuclear™ Transcription Factor Buffer Set	Biolegend	424401

1	Penicillin–Streptomycin-Glutamine (100X)	Gibco	10378016
2	FBS	GIBCO	10099–141
5	DMEM	GIBCO	10566–016
6	Opti-MEM	Gibco	31985070
7	Trypsin–EDTA (0.25%), phenol red	Gibco	25200072
8	InVivoMab anti-mouse CD4	BioXcell	BE0003-1-5MG
9	InVivoMab anti-mouse CD8	BioXcell	BE0004-1-5MG
10	100um cell strainer	Falcon	352360
11	70um cell strainer	Falcon	352350
12	Percoll	GE Health	17–0891-02
13	DPBS	Hyclone	SH30028.02
14	TLRL-1826 ODN	Invivogen	tlrl-1826–1
15	Dimethyl sulfoxide (DMSO)	Sigma-Aldrich	D2650
16	Collagenase A	Roche	10103586001
17	HBSS	Servicebio	G4204-500
18	10XPBS	Servicebio	G4207-500
19	mouse IFN-gamma elispot plus(plus) HRP	MabTech	3321-4HST-2
20	Bovine Serum Albumin	SIGMA	V900933-100G
21	mouse IFN-gamma elispot plus(plus) HRP	MabTech	3321-4HST-2
22	CD45 MicroBeads, mouse	Miltenyi Biotec	130–052-301
23	Mouse IFN-gamma ELISpot PLUS (HRP), strips	MabTech	3321-4HST-10
24	EmbryoMax™ Nucleosides (100X)	merck	ES-008