FIG. 1.

catA mRNA accumulation and catalase activity during growth and conidiation in wild-type and brlA1 mutant strains. (A) Total RNA extracted from growing mycelia (0 h of conidiation), mycelia induced to conidiate for 25 h, or isolated conidia were fractionated in formaldehyde-agarose gels, transferred to a nylon membrane, and hybridized to the catA PstI fragment from pCAN5 and an actin-specific probe from pSF5. (B) Cell-free soluble protein extracts, prepared from samples at 0 and 25 h of conidiation, isolated conidia (20 μg), or ascospores (40 μg), were separated in a native polyacrylamide gel and stained for catalase activity (27). Samples corresponding to strains FGSC-26 (wild type), AJC7.1 (brlA1), and CRN6 (brlA17 catA) are indicated. Catalase A and B positions are shown by arrows.