FIG. 5.

catA mRNA accumulation during different stress conditions. Mycelia from strain TLK12, grown for 12 h in minimal medium at 37°C, were transferred to minimal medium and subjected to the following treatments: lanes 1 and 2, 2 and 3 h in minimal medium, respectively (controls); lane 3, 5 mM paraquat for 2 h; lane 4, 0.5 mM hydrogen peroxide for 2 h; lane 5, 0.8 mg of uric acid per ml as the sole nitrogen source for 2 h; lane 6, 42°C for 3 h; lane 7, 1 M sorbitol for 3 h; lane 8, 1 M sodium chloride for 3 h; lane 9, 4% ethanol as the sole carbon source for 3 h; lane 10, minimal medium lacking glucose for 3 h; lane 11, minimal medium lacking nitrate for 3 h. Total RNA from the indicated conditions was fractionated in formaldehyde-agarose gels, transferred to a nylon membrane, and hybridized to a catA-specific probe. The same membrane was hybridized to an actin probe as a loading control.