FIG. 6.

catA mRNA and catalase activity accumulation during starvation-induced submerged sporulation. Strain TRN3 was grown for 18 h in glucose medium and shifted to standard medium (mm + C + N) or medium lacking glucose (mm − C). Samples taken at 24 h were filtered through Miracloth to retain mycelia. The resulting filtrate was passed and rinsed through 0.22-μm-pore-size Millipore membranes to collect the conidia produced during starvation. (A) Total RNA obtained from starved mycelia was subjected to Northern blot analysis using a catA- or actin-specific probe. (B) Corresponding protein extracts were fractionated in a native polyacrylamide gel and used to determine catalase activity (27).