Skip to main content
. 2023 Oct 10;226(1):iyad182. doi: 10.1093/genetics/iyad182

Fig. 3.

Fig. 3.

Hippocampal (HC) pathology in Nes-cre, ex7−/− mutants. a) Dentate gyrus (DG) granule cells were more densely packed in P14 Nes-cre, ex7−/− HC as compared to WT, but CA1 pyramidal cells were normally packed. N = 30–53 counts on matching sections from 3–6 mice for each genotype. b) DG structure thickness was significantly decreased in P14 Nes-cre, ex7−/− mice compared to WT animals, although CA1 structure thickness was not significantly different in WT and mutant mice. N = 28–50 measurements on matching sections from 3–6 mice for each genotype. c) The total number of cells per column in the DG was significantly reduced in both P14 and adult Nes-cre, ex7−/− HC compared to WT littermates. N = 28–36 counts for P14 and 13–20 counts for adult on matching sections from 3 mice for each genotype. d) The ratio of CalB+/CalR+ cells was significantly decreased in Nes-cre, ex7−/− mutants DG compared to WT littermates. N = 28–36 counts on matching sections from 3 mice for each genotype. e) Representative picture of the CalB+ and CalR+ layers in DG of P14 WT and Nes-cre, ex7−/− HC. The upper box highlights the CalB+ cell layer and the lower box shows CalR+ cell layer in each sample. f) The thickness of the suprapyramidal bundle of the DG gc-CA3 projection was significantly reduced in P14 and adult Nes-cre, ex7−/− animals compared to WT HC. Three measurements were averaged for each section examined. N = 6 sections for P14 and 10–11 sections for adult from 3 mice for each genotype. g) The number of TBR2+ cells was significantly reduced in the hilus and DG gc layer (Hi + gcl) and increased in molecular layer (ML) of the DG in P14 Nes-cre, ex7−/− HC compared to WT controls (Supplementary Fig. 2a), although the total number of TBR2+ cells was not significantly different between WT and mutant animals. N = 12–13 matching sections from 3 mice for each genotype. h) HMN collateral axon tracks stained with CalR show a bright signal and normal thickness in adult Nes-cre, ex7−/− HC. Two-tailed t-test was used to test statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = not significant.