Fig. 6.

Behavioral characterization of Cmv-cre, ex7^−/+ animals, abbreviated as ex7^−/+ in figures. a) Novel object recognition test showed that ex7^−/+ animals displayed normal exploratory activity (left panel) and cognitive memory (right panel) compared to WT animals. N = 9–16 for exploratory activity and 9–13 for cognitive memory for each genotype. One outlier was excluded from ex7^−/+ group in exploratory activity test with Grubb's test, G = 2.903 at alpha = 0.05. b) Three-chamber test (see Methods) showed that ex7^−/+ animals displayed normal sociability (left panel) and social memory (right panel) compared to WT animals. N = 8 for sociability and 6 for social memories for each genotype. c) Developmental delay tests of righting reflex and eye-opening (EOP) scores (see Methods). Ex7^−/+ mice showed normal establishment of righting reflex but reduced scores in EOP test compared to WT mice N = 10–14 for righting reflex measurement and 12–13 for EOP test for each genotype. d) Body weight measurements at different developmental time points. P5–P60 animals were measured for body weight. WT N = 8–17; ex7^−/+ N = 9–15. e) Stereotypic behavior was measured with grooming time per episode and significantly increased in ex7^−/+ animals. N = 11 for each genotype. f) Horizontal activity was significantly increased in ex7^−/+ animals compared to WT controls. N = 9–16 for each genotype. Two-tailed t-test was used to determine statistical significance; *P < 0.05, **P < 0.01, ****P < 0.0001, ns = not significant (P > 0.05).