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AgpA is a periplasmic protein needed for transport of the α-galactoside raffinose. (A) Periplasmic proteins from strains Rm8002 (agpA+) and SG1001 (agpA) grown in M9 glucose, which represses agpA expression, or in M9 galactose, which induces agpA expression. (B) Transport of raffinose by strains Rm8002 and SG1001. Raffinose uptake assays were performed with cells from the cultures used for isolating the periplasmic proteins shown in panel A.
