Fig. 2.

Hsa_circ_001726 deficiency inhibited HCC progression in vivo and in vitro. A QRT-PCR detected the expression of hsa_circ_001726 in adjacent nontumor tissues tumor tissues of HCC patients with or without metastasis. B QRT-PCR detected the expression of hsa_circ_001726 in LO2, Sk-Hep1, Bel7402, MHCC97H and Huh7 cells. C, D Wound healing and Transwell invasion assays examined migration and invasion of MHCC97H and Huh7 cells following transfection of si-hsa_circ_001726 or si-NC. Xenograft mouse model was constructed by inoculating Huh7 cells with LV-sh-hsa_circ_001726 or Huh7 cells with LV-sh-NC. E–G Tumor volume and weight of xenograft mice. Orthotopic transplantation tumor model was constructed by inoculating Huh7 cells with LV-sh-hsa_circ_001726 or Huh7 cells with LV-sh-NC. H The images of lung tissues. I, J HE staining examined the pathological changes of lung tissues. ^*P < 0.05, ^**P < 0.01 vs LO2, si-NC or sh-NC group