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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Nat Chem Biol. 2023 Jun 15;19(8):1004–1012. doi: 10.1038/s41589-023-01318-1

Extended Date Figure 6. Copy strand synthesis improves DNA carboxymethylation efficiency.

Extended Date Figure 6.

a) Experimental scheme. Sheared lambda gDNA is ligated to 5mC- or 5pyC-containing adapters. A copy strand with 5mCs is synthesized before reaction with the CxMTase and either no SAM, SAM, or CxSAM, with the product of this reaction represented by the oval with mixed colors. Subsequent BS or A3A deamination shows efficiency of DNA modification. Data are presented as mean values +/− SD (n=2 independent experiments). b) Next-Generation Sequencing quantifying efficiency of CxMTase with methylation or carboxymethylation after copy strand synthesis.