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. 2021 Oct 27;7(44):eabi5797. doi: 10.1126/sciadv.abi5797

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Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 6. — (A) CDC34A~ubiquitin discharge. (B) UBE2R2 thioester discharge. Duplicate blots were probed with either anti-CDC34A, anti-UBE2R2, or, as a loading control, anti-tubulin. Asterisks indicate residual E2-thioester species in reducing (+DTT) conditions. 4924 indicates MLN4924.