Table 2.
Virus shedding of chickens challenged with H5N1-D889 virus.
| Group | Challenge virus | Oropharyngeal swab (virus shedding number/total number) | Cloacal swab (virus shedding number/total number) | Total virus shedding number/total number | ||||
|---|---|---|---|---|---|---|---|---|
| 3 dpca | 5 dpc | 7 dpc | 3 dpc | 5 dpc | 7 dpc | |||
| H5N1VLP + ISA 71 (60 µg) | D889 | 0/10 | 0/10 | 0/10 | 0/10 | 1/10 | 2/10 | 2/10 |
| H5N1VLP + ISA 71 (80 µg) | D889 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 | 0/10 |
| Commercial vaccine | D889 | 0/10 | 0/10 | 0/10 | 0/10 | 1/10 | 1/10 | 1/10 |
| PBS | D889 | NAb | NA | NA | NA | NA | NA | NA |
Chickens were inoculated intranasally challenged with 106.0 EID50 of H5N1-D889 virus. The oropharyngeal and cloacal swab samples were collected at 3, 5, and 7 days post-challenge. Virus positivity or shedding was determined by inoculating each swab solution into 3 eggs of 10-day-old specific-pathogen-free chicken embryos. adpc, days post-challenge. bNA, not applicable due to the death of chickens.