Table 1.
Validation results of significantly regulated genes in PPD-stimulated PBMCs between HHC-1 and HHC-23 group in a test set by qRT-PCR.
| GO terms | Gene symbol | Median fold change | Ratio | P-value | |
|---|---|---|---|---|---|
| HHC-23 | HHC-1 | ||||
| IL-17 signaling pathway | CXCL3 | 10.88 | 3.16 | 3.44 | <0.0001 |
| NFKBIA | 8.46 | 7.89 | 1.07 | 0.135 | |
| CXCL2 | 11.11 | 10.30 | 1.08 | 0.035 | |
| IL8 | 12.85 | 10.59 | 1.21 | 0.009 | |
| CSF2 | 5.89 | 4.38 | 1.34 | 0.006 | |
| Complement and coagulation cascades | SERPINA1 | 7.11 | 6.27 | 1.13 | 0.011 |
| PLAUR | 7.85 | 7.23 | 1.09 | 0.043 | |
| SERPINE1 | 0.80 | 1.51 | 0.53 | 0.161 | |
| PROCR | 3.59 | 2.60 | 1.38 | 0.022 | |
| SERPINB2 | 10.47 | 9.58 | 1.09 | 0.014 | |
| Ferroptosis | ACSL5 | 6.73 | 6.14 | 1.10 | 0.035 |
| ATG7 | 4.42 | 2.54 | 1.74 | <0.0001 | |
| MAP1LC3A | 2.79 | 2.98 | 0.94 | 0.160 | |
| SLC3A2 | 5.89 | 5.97 | 0.99 | 0.257 | |
| FTL | 12.37 | 11.48 | 1.08 | 0.002 | |
| TNF signaling pathway | TNFRSF1B | 3.81 | 8.24 | 0.46 | <0.0001 |
| CCl20 | 9.41 | 10.95 | 0.86 | 0.004 | |
| MMP14 | 7.42 | 8.08 | 0.92 | 0.001 | |
| ICAM1 | 6.39 | 7.11 | 0.90 | 0.016 | |
| SOCS3 | 5.74 | 5.31 | 1.08 | 0.012 | |
Fold change: the fold change was calculated by dividing gene expression level of PPD-stimulated sample by gene expression level of unstimulated sample by qRT-PCR.
Ratio: the ratio was calculated by dividing mean fold change of one group by mean fold change of another group.