TcapQ activation in vivo. In vivo images were taken in a rat eye pretreated with 12.5 mM NMDA immediately before (A) and at 4 hours (B), 12 hours (C), 25 hours (D), 48 hours (E), and 72 hours (F) postintravitreal injection of 0.313 nmol TcapQ488. Evidence of initial probe activation was noted at 4 hours after TcapQ488 injection (B). Scale bar, 200 μm in all images. Reproduced from Qiu et al.38