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. 2020 Nov 13;6(46):eaba5723. doi: 10.1126/sciadv.aba5723

Fig. 1. Characterization and antibacterial performance of L. casei biofilm.

Fig. 1

(A) General concept of using inactivated L. casei biofilm to prevent MRSA infection and improve bone tissue regeneration simultaneously. (B) Surface morphology of L. casei biofilm–modified AHT-Ti. (C) Live/dead (green/red) staining of L. casei biofilm–modified AHT-Ti. (D) Section image of L. casei-AHT-Ti. (E) Growth of MRSA on the surface of Ti, AHT-Ti, and L. casei-AHT-Ti and corresponding photos of bacteria solution after 12 hours (right). Photo credit: L.T., Hubei University. (F) Number of MRSA colonies in the groups of Ti, AHT-Ti, L. casei-AHT-Ti-1 (1 day), L. casei-AHT-Ti-2 (2 days), and L. casei-AHT-Ti (3 days). (G) Lactic acid and (H) H2O2 detections from L. casei-AHT-Ti-1, L. casei-AHT-Ti-2, and L. casei-AHT-Ti with different culture time of biofilm. (I) SEM images of bacteria on the surface of samples. n = 3 independent experiments per group, *P < 0.05, **P < 0.01, and ***P < 0.001. ns, not significant.