FIG. 5.

Primer extension analysis of PureIΔ100CAT. Total cellular RNA of S. salivarius PureIΔ100CAT from a steady-state culture grown at pH 5.5 with 20 mM glucose was isolated, and the radiolabeled primer cat (5′-AATGCCTCAAAATGT-3′) was used. The DNA sequences were derived from pMC71 with the same primer.