Table 1.

Publications reporting GCE-based dual-ncAA encoding that are identified in this review. Entries indicated by an “*” perform both dual encoding and dual labeling, “†” indicates that meta-azidophenylalanine (mAzF), a meta-substituted isomer of pAzF (C) was used.

		Subsystem A			Subsystem B			Subsystem C			System D
Entry	Protein(s)	ncAA(s)	Codon	aaRS/tRNA	ncAA(s)	Codon	aaRS/tRNA	ncAA	Codon	aaRS/tRNA	ncAA	Codon	aaRS/tRNA	Reference
Prokaryotic Systems
1	Myoglobin	OMeY	UAG	MjTyr	hGln	AGGA	PhLys							Anderson et al., 2004182
2*	Calmodulin	pAzF (C)	AGGA	MjTyr	PrK (A) BocK	UAG	MbPyl							Neumann et al., 201034
3	GFPuv	pAzF (C)	UAG	MjTyr	BocK PrK (A) CycpK	UAA	MbPyl							Wan et al., 201090
4*	QBP	pAzF	UAG	MjTyr	KetoK (F)	UAA	MmPyl							Wu et al., 201228
5*	Calmodulin	pAcF (G)	UAG	MjTyr	PrK (A)	UGA	MmPyl							Kim et al., 201326
6*	KSI GFP	pAzF (C) pAcF (G)	UAG	MjTyr	AzK (D) BocK	UAA	MbPyl							Chatterjee et al., 201318
7*	GST-CaM	pAzF (C) Tet1.0 (H) PrY (B) Bpa	UAG AGGA	MjTyr	BocK PrK (A) AlkK NbK (L)	UAG AGUA UAGA	MbPyl							Wang et al., 201417
8*	Calmodulin	PrY (B)	UAG	MjTyr	CpK (M)	AGUA	MbPyl							Sachdeva et al., 201491
9	GFP GST-MBP	pAzF (C)	AGGA	MjTyr	PrK (A) BocK	UAG	MbPyl							Lammers et al., 201427
10	sfGFP MDH	Sep	UAG	MmSep	AcK (D)	UAA	MbPyl							Venkat et al., 2018125
11	QBP	pAzF (C)	UAG	MjTyr	PrK (A)	UAA	MmPyl							Tharp and Liu, 201896
12	sfGFP Histone H3	Bpa	UGA	MjTyr	AcK	UAG	MbPyl							Zheng et al., 201892
13	GST-Calmodulin	CbzK	UAG	MaPyl	CpK (M)	AGGA	MmPyl							Willis and Chin, 2018103
14	TrxR1	AcK	UAG	MbPyl	Sec	UGA	EcSec							Wright et al., 2018183
15*	sfGFP Herceptin-Fab	pAzF (C) OMeY	UAG	MjTyr	CpK (M) BocK	UAA	MbPyl	5HTP (N)	UGA	EcTrp				Italia et al., 201972
16	GFPuv	pAcF (G)	UAGA	MjTyr	BocK PrK (A)	AGGA	MbPyl							Hankore et al., 201993
17	ScFv-P3	PrY (B)	UAG	MjTyr	CpK (M)	AGUA	MbPyl							Oller-Salvia et al., 201994
18	sfGFP	pAzF (C)	AXC	MjTyr	AzK (D)	AGX	MmPyl	N/A	GXT	N/A				Fischer et al., 202097
19	GFP	BocK	UAG	MmPyl	NmH	AGGA	Lum1Pyl	CbzK	AGUA	1R26Pyl				Dunkelmann et al., 2020105
20*	sfGFP	MeF	UAG	MjTyr	BocK	UAA	MaPyl							Tharp et al., 2020130
21*	tsCA	pAzF (C)	UAG	MjTyr	Tet3.0 (I)	UAA	MbPyl							Sosa et al., 202195
22	Ubiquitin Noncanonical polymer	pIF	UAG	AfTyr	CbzK	UCG	1R26Pyl	BocK AllocK CpK (M) PrK (A)	UCA	MmPyl				Robertson et al., 2021136
					AllocK PrK (A)	UCG	MmPyl	CbzK	UCA	1R26Pyl
23	Strep-GFP	NmH	AGGA	RumEnPyl	CbzK	AGUA	G1Pyl	AllocK	UAGA	MmPyl	pIF	CUAG	AfTyr	Dunkelmann et al., 2021131
24*	sfGFP SUMO-sfGFP sfGFP-mTagBFP2 scRPA	pAzF (C)	UAG	MjTyr	Tet3.0 (I)	UAA	MbPyl							Bednar et al., 20211
25*	sfGFP	mAzF (C†)	UAG	MaPyl	PrK (A)	UAA	MmPyl	pAcF	UAU	MjTyr				Tharp et al., 202198
Eukaryotic Systems
26*	EGFP Anti-HER2 IgG	pAcF (G) OMeY	UAG	EcTyr	AzK (D) BocK	UAA	MbPyl							Xiao et al., 201330
27	CRF1R	pAzF (C)	UAG	EcTyr	CbzK	UGA	MbPyl							Serfling et al., 2017184
28	EGFP	OMeY pAzF (C) PrY (B) pAcF (G)	UGA UAG	EcTyr	BocK AzK (D) PrK (A) CpK (M)	UGA UAG	MbPyl						Zheng et al., 201799
		Cap	UAG	EcLeu
29	sfGFP	TCOK (J)	UAG	MaPyl	AcK	UAA	MmPyl							Meineke et al., 201880
30	EGFP	C5Az (E)	UAG	EcLeu	CpK (M) NbK (L)	UGA	MbPyl							Zheng et al., 201879
31	GFP	NmH	UGA	MaPyl	BocK	UAG	MmPyl							Beranek et al., 2019104
32*	CRF1R SynNotch sfGFP	PrK (A)	UAG	G1Pyl	TCOK (J)	UAA	MmPyl							Meineke et al., 202081
33	GFP	Anap	UAG	EcLeu	pAcF	UAA	EcTyr	AzK (D)	UGA	MmPyl				Shi et al., 2022100