Fig. 1.

Quantification of circulating monocytes in sepsis.

a. The first two principal components of the clustering results using all 8267 genes present in the pooling whole blood leukocyte profiling before (up) and after (down) batch effect correction. b. Heatmap of the landscape of both NESs calculated based on distinct monocyte reference gene sets and NRFs generated by deconvolutional methods of monocytes. c, d. Distribution of the NES (c) and NRF (d) of monocytes exhibited a decline in the septic patients compared with healthy controls. Two-tailed Student's t-test was performed in c and d. ^nsp > 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Abbreviations: PC: principal component, HC: healthy control, S: septic patient, NES: normalized enrichment score, NRF: normalized relative fraction.