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. 2023 Dec 21;10:1272835. doi: 10.3389/fvets.2023.1272835

Table 4.

Rumen methanogens diversity and taxa abundances in 26-week-old goats measured at 21 days after a shift from a high-concentrate to a full forage diet (oats hay).

Treatments 1 CTL AUT RFF RFC SED p-value
Concentration, log10 copy/mg DM 6.60 6.85 6.83 7.24 0.343 0.218
Diversity
Richness, OTUs 8.67b 9.00b 13.5a 11.0ab 1.452 0.008
Shannon 1.32a 0.92b 1.37a 1.32a 0.128 0.005
Simpson 0.66a 0.47b 0.62a 0.64a 0.056 0.009
Abundance 1, %
f_Methanobacteriaceae 17.4 22.2 26.4 23.6 3.768 0.764
f_Methanocaldococcaceae 0.00 0.00 0.15 0.28 0.091 0.187
g_Methanocaldococcus 0.00 0.00 0.00 0.28 0.077 0.058
f_Methanomicrobiaceae 3.73 0.00 0.00 3.57 1.657 0.534
f_Methanomassiliicoccaceae 78.9 77.8 73.2 72.6 3.639 0.838
s_Group8_sp WGK1 0.00 0.05 1.81 0.71 0.521 0.088
s_Group10_sp 18.5 3.99 4.63 6.34 2.446 0.053
s_Group11_sp ISO4-G11 19.1a 0.00b 18.8a 18.5a 4.697 0.018
g_Group9 34.5b 71.4a 43.8ab 40.4ab 6.052 0.041
s_Group9_sp CH1270 0.00b 0.00b 0.01b 0.43a 0.125 0.046
s_Group9_sp ISO4-G1 34.3b 71.4a 43.8ab 40.0ab 6.049 0.035
1

During the first 10 weeks of age, goats (N = 36) received a daily and oral inoculation with autoclaved rumen fluid (AUT), rumen fluid from adult animals fed forage (RFF), or fed concentrate diets (RFC) or without inoculation (CTL). Means within a raw with different superscript differ (p < 0.05).