Fig. 1.

DOT1L is highly detected in dialysate effluent of long-term PD patients and mainly expressed in peritoneal cells with fibrotic phenotype, regulating the development of peritoneal fibrosis induced by PDF in mice. a Violin plot showed the expression levels of DOT1L, TGF-β1, VEGF, MMP2 and CA125 in peritoneal dialysis effluent according to ELISA. These patients were divided into three groups according to duration, Group I duration < 12 months (n = 29), Group II 12 months ≤ duration < 36 months (n = 25) and Group III 36 months ≤ duration (n = 18). b Correlations between DOT1L and levels of CA125, TGF-β1, VEGF and MMP2 in PD effluent. c, d Co-immunofluorescence photomicrographs illustrated co-staining of DOT1L and α-SMA in the peritoneum from patient and mouse with peritoneal fibrosis induced by PDF. Arrows indicated α-SMA-positive cells. e Photomicrographs showed Masson’s trichrome staining of the peritoneum in each group. f the bar graph showed the positive area of the Masson-positive submesothelial area (blue) from 10 random fields of peritoneal samples from six mice. g the bar graph showed the thickness of the compact zone measured from 10 random fields of peritoneal samples from six mice. h the D/P ratio of BUN. i the D/D0 ratio of glucose. Scale bar = 50μm. Data are means ± sem of 6 samples, *P < 0.05, **P < 0.01, *** P < 0.001, P ≥ 0.05 is not significant (NS)