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. 2024 Jan 4;5:3. doi: 10.1186/s43556-023-00161-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Mesothelial cells crosstalk with macrophages by secreting cytokines. a Raw264.7 cells were cultured with 10% (vol/vol) pre-collected cell culture media from HPMCs with different treatment. b Serum-starved HPMCs were cultured in 60mM high glucose for 36 h with different concentrations of EPZ5676 (0, 1, 5, 10 μM). Bar graphs showed the expression levels of EGF, TGF-β1 and IL-4 in media from HPMCs according to ELISA. c Serum-starved HPMCs were pretreated with scramble siRNA or DOT1L siRNA and then exposed to 60mM high glucose for an additional 36 h. Bar graphs showed the expression levels of EGF, TGF-β1 and IL-4 in media from HPMCs according to ELISA. d Immunofluorescence of CD163 in Raw264.7 cultured with different media. e Regulatory mechanism of DOT1L in peritoneal fibrosis. PD related stimulus induces DOT1L upregulation, which promotes the expression and activation of tyrosine kinases EGFR in mesothelial cells and JAK3 in macrophages, promoting cells differentiate into fibrotic phenotype, deposition of ECM and thus peritoneal fibrosis. Scale bar = 50μm. Data are means ± sem of 4 samples, *P < 0.05, **P < 0.01, *** P < 0.001, P ≥ 0.05 is not significant (NS)