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. 2024 Jan 3;14:308. doi: 10.1038/s41598-023-51091-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of antioxidant capacity by methods (A) ORAC, (B) FRAP and (C) DPPH. The pollen extract and the flavonoid quercetin were diluted in methanol, while the ascorbate was diluted in water. Samples were diluted to 1 mg/mL for the FRAP assay and serially diluted from that concentration for the DPPH assay. For the ORAC method, samples were diluted to 100 μg/mL. Values were expressed as percent inhibition (%) for the DPPH method, with IC50 given as µg/mL, and as µmol trolox/g sample equivalents for ORAC and FRAP. Values expressed as mean ± standard error. Different letters indicate non-significant difference when compared to the control (Ascorbate) (P < 0.05).