FIG. 1.

Restriction map of the region downstream from the rmlD gene of S. mutans Xc. The arrows indicate the locations of the eight ORFs including the carboxy-terminal half of rmlD. The pResYT10 integration sites for the insertional inactivation of these ORFs are indicated by the inverted open triangles. The locations of the insertional fragments are indicated in the lower portion of the diagram. Restriction enzyme abbreviations and primer sequences are as follows: Ba, BamHI; Ec, EcoRI; Ps, PstI; Sp, SpeI; 1F, 5′-ATGGTCACGTCCAGTGCA-3′; 1R, 5′-GTCCAATACCGTGCAGCA-3′; 2F, 5′-AGCTAAGCAGTGGAAGCAG-3′; 2R, 5′-CTGCATCCCACTAGAATAG-3′; 3F, 5′-GAAGCTGGATTGGTGAAGC-3′; 3R, 5′-TGTAGGAATGGTCCAACG-3′; 4F, 5′-AGGTGATTGACCAGTATG-3′; 4R, 5′-ATCTGTCACTAGCAGAGG-3′; 5F, 5′-TGTCCACCTACAATGGTC-3′; and 5R, 5′-GAAAGGCACGATTCTTAG-3′.