Figure 3.

Survival curves of the force-fed larvae (a) and CFU counts of the bacteria that had colonized after 24 h and 48 h of last force-feeding (b) with increasing doses of Kp14520. (a) Larvae were force-fed at 0 h, 24 h, and 48 h, and the bacterial doses are indicated in the legend. The survival of the larvae was noted at 24 h, 48 h, and 72 h post force feeding. Each experiment was repeated thrice with 20 larvae per group (n = 60), and the data were analyzed with the log-rank (Mantel–Cox) test (results depicted in Table S1). (b) Box and whisker plot of the gut colonization of the different larval groups after 24 h and 48 h of last force-feeding. The Gram-negative bacteria were selected on MacConkey agar. The experiment was performed thrice, with six larvae per group on the first attempt and ten larvae per group on the second and third attempts (n = 26). The data were analyzed with the Kruskal‒Wallis test (the results are shown in Table S1). The boxes represent the 90th-10th percentiles, the line represents the median, + represents the mean and dots represent values outside the percentile.