FIG. 6.

Effects of Zn and EDTA on growth of wild-type (WT) and yciC, ycdH, and yciC ycdH mutant strains. (A) Cell optical density (OD) after overnight growth in LZMM (light bars) or LZMM supplemented with 10 μM Zn²⁺ (dark bars) is indicated for each strain. The results are representative of experiments performed three or more times. (B) Growth inhibition by EDTA. For each strain, the diameter of the zone of growth inhibition was determined on MM plates surrounding a 0.6-cm-diameter filter paper disk containing 10 μl of EDTA at either 10, 100, or 500 mM. Strains are wild type (○), yciC (•), ycdH (□), and yciC ycdH (▴). Values represent the overall diameter of inhibition minus the diameter of the filter and are reproducible to within 0.2 cm.