Figure 1.

Generation and characterization of the INS^{Cherry/Cherry}(INS^Δ123/Δ123) reporter iPSC line. A) Schematic picture of stepwise pancreatic endocrine lineage formation. B) Schematic representation of proinsulin mRNA and the expected translated and processed protein from WT INS and INS-T2A-H2B-Cherry alleles. C) INS and H2B-Cherry expression in INS^Cherry/WT and INS^{Cherry/Cherry} SC-islets. Scale bar, 50 μm. D) INS and C-peptide expression in INS^Cherry/WT and INS^{Cherry/Cherry} SC-islets. Scale bar, 50 μm. E) Western blot showing the expression of proinsulin in INS^Cherry/WT and INS^{Cherry/Cherry} SC-islets from S5.3 to S6.10. F) Total insulin from INS^Cherry/WT vs INS^{Cherry/Cherry} SC-islets at S6.21. G) qPCR analysis of INS mRNA expression in SC-islets at S5.7. H) Schematic picture of 123 base pair (bp) deletion in exon 3 of INS mRNA from INS^{Cherry/Cherry} cell line, and the expected translated peptides. Schemes were created with BioRender.com. Data are represented as mean ± SD.