Figure 2.

Characteristics of SC-β cells derived from the INS^Δ123/Δ123iPSC line. A) Representative immunofluorescence pictures showing the presence of cells co-expressing PDX1, NKX6-1 and H2B-Cherry in SC-islets at S6.14. Scale bar, 20 μm. B-G) Electron microscopy imaging depicting WT and INS^Cherry/WT SC-β cells containing mature SGs (yellow arrowheads) and TSGs (green arrowheads) and INS^Δ123/Δ123 SC-β-cells containing low-dense SGs with scattered electron density (red arrowheads). Immature SGs (blue arrowheads) and vesicles with very low/no electron density (purple arrowheads) are also shown. Scale bars: 1 μm (for inserts 2 μm).