Figure 5.

Insulin shortage alters human endocrine cell composition. A) Representative FACS plots of INS⁺ and H2B-Cherry⁺ cells at S5.7. B) FACS quantification of INS⁺ cells, relative to the total endocrine cell population at S5.7. C) Representative FACS plots of GCG⁺ and H2B-Cherry⁺ cells at S5.7. D, E) FACS quantification of H2B-Cherry⁺ and GCG⁺ cells, relative to the total endocrine cell population at S5.7. F) Ratio of GCG⁺ to H2B-Cherry⁺ at S5.7. G) FACS quantification of GCG⁺-H2B-Cherry⁺ cells, relative to the total endocrine cell population at S5.7. H) Immunofluorescence pictures showing the H2B-Cherry⁺ and GCG⁺ cells at S5.7. Scale bar, 80 μm. I) Quantification of the percentage of H2B-Cherry⁺, GCG⁺ and GCG⁺-H2B-Cherry⁺ cells to the total endocrine cells, as well as the ratio of GCG⁺ to H2B-Cherry⁺ cells at S5.7. J) Representative FACS plots of INS⁺ and H2B-Cherry⁺ cells in SC-islets at S6.11. K) Representative FACS plots of GCG⁺ and H2B-Cherry⁺ cells in SC-islets at S6.11. L) Quantification of H2B-Cherry⁺, GCG⁺ and GCG⁺-H2B-Cherry⁺ cells, as well as the ratio of GCG⁺ to H2B-Cherry⁺ cells in SC-islets at S6.11. M) Immunofluorescence pictures showing the H2B-Cherry⁺ and GCG⁺ cells in the SC-islets at S6.11. Scale bar, 80 μm. Data are normalized to INS^Cherry/WT. All statistics have been done using two-sided unpaired t-test. Data are represented as mean ± SD.