Supplementary figure 6. Reinstalling insulin signaling restores endocrine cell composition in INS^Δ123/Δ123 SC-islets. a) Schematic picture depicting that the sorted and enriched SC-β cells from differentiation (diff.) 1 were mixed with progenitors from diff. 2 at ratio of 30:70 % and the differentiation proceeds for 7 days. b) Live microscopy imaging of H2B-Cherry⁺ cells in INS^Cherry/WT SC-β cells reaggregated with INS^Cherry/WT progenitors (Cherry:Cherry), INS^Δ123/Δ123 SC-β with INS^Δ123/Δ123 progenitors (Δ123:Δ123) and INS^Cherry/WT SC-β with INS^Δ123/Δ123 progenitors (Cherry:Δ123). An increased Cherry signals in the Δ123:Δ123 mixture is observed compared to the Cherry:Cherry condition. Notably, the Cherry:Δ123 mixture exhibits reduced Cherry signals at the levels of the Cherry:Cherry culture. Scale bar, 300 μm. c) Representative FACS plots of INS⁺ and H2B-Cherry⁺ cells in Cherry:Cherry, Δ123:Δ123 and Cherry:Δ123 SC-islets. d) FACS quantification of total INS⁺ cells. Data are normalized to Δ123:Δ123. e) FACS quantification of H2B-Cherry⁺ cells to total cells. Data are normalized to Δ123:Δ123. f) Representative FACS plots of GCG⁺ and H2B-Cherry⁺ cells. g) FACS quantification of GCG⁺ cells to total cells. Data are normalized to Cherry:Δ123. h) FACS quantification of the ratio of GCG⁺ cells to H2B-Cherry⁺ cells. Data are normalized to Cherry:Cherry. i) FACS quantification of GCG⁺-H2B-Cherry⁺ cells to total cells. Data are normalized to Cherry:Δ123. Schemes were created with BioRender.com. All statistics have been done using one-way ANOVA and Tukey's multiple comparisons test. Data are represented as mean ± SD.