Skip to main content
. 2023 Nov 27;63(1):9–18. doi: 10.1021/acs.biochem.3c00433

Figure 2.

Figure 2

(A) Digest assay comparison between MBP and random substrates: (1) casein, (2) MBP, (3) α-lactalbumin, and (4) lysozyme; each set shows +/− Abs from ME/CFS_11 patient, (B) kinetics of ME/CFS_11 Abs showing correlation/percent relative activity (% RA) of MBP breakdown with increasing Ab concentration, and (C) PAGE image of ME/CFS_11 Ab with (I) nonreducing and (II) reducing conditions after protein-A purification to demonstrate Ab purity; first lane is a protein ladder. Further densitometry analysis of the digest assay (A) comparing the MBP bands +/– Ab showed the target full-length product (∼18,400 Da) for each to be 687 and 1305 (dpi), respectively. All substrates (+/– Ab) were incubated at 37 °C for 24 h.