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. 2023 Nov 27;63(1):9–18. doi: 10.1021/acs.biochem.3c00433

Figure 3.

Figure 3

(A) Digest assay with ME/CFS_11 after HPLC fraction-collection of intact Ab using the Superdex-200 column. After protein-A purification, the sample was processed with HPLC and fraction-collected in six fragments (the chromatogram of Ab sample shows vertical bars separating each fraction corresponding to PAGE image of the postdigest assays above). (B) Digest assay following affinity purification of total Abs collected from ME/CFS_15 plasma sample; lane 1 shows unbound nonspecific Abs from the wash step, and lane 2 shows MBP-specific Abs captured and eluted from the resin conjugated with MBP.