KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bodipy | ThermoFisher Scientific | D3922 |
| PHA | Cytoskeleton | PHDH1-A |
| PE anti-THY1 | BioLegend | Cat# 105308; RRID: AB_313179 |
| BV605 anti-LY6A | BioLegend | Cat# 108133; RRID: AB_2562275 |
| AF700-CD24 | BioLegend | Cat# 101836; RRID: AB_2566730 |
| FITC anti-mCD26(DPP4) | Biolegend | Cat# 137806; RRID: AB_10663402 |
| APC anti-PDGFRA | eBioscience | Cat# 17140181; RRID: AB_529482 |
| PECy7 anti-CD45 | Biolegend | Cat# 147704; RRID: AB_2563536 |
| PerCP-Cy5.5 anti-CD31 | Biolegend | Cat# 102522; RRID: AB_2566761 |
| FITC anti-Ly6G | eBioscience | Cat# 11593182; RRID: AB_465314 |
| PECy7 anti-CD11B | Biolegend | Cat# 101216; RRID: AB_312799 |
| AF488 anti-SMA | eBioscience | Cat# 53976082; RRID: AB_2574461 |
| PE anti-F4/80 | eBioscience | Cat# 12480182; RRID: AB_465923 |
| PerCP-Cy5.5 anti-Ly6C | BioLegend | Cat# 128012; RRID: 1659241 |
| APC anti-CD11C | BioLegend | Cat# 117310; RRID: AB_313779 |
| AF700 anti-MHCII | eBioscience | Cat# 56532182; RRID: AB_494009 |
| APC-Cy7 anti-CD3 | BioLegend | Cat# 100222; RRID: AB_2242784 |
| Goat anti-PLIN | Abcam | Cat# AB61682; RRID: AB_944751 |
| Rat anti-CD24 | BioLegend | Cat# 101801; RRID: AB_312834 |
| Goat anti-PDGFRA | R&D system | Cat# AF1062-SP ; RRID: AB_2236897 |
| Rabbit anti-THY1 | Cell Signaling Technology | Cat# 13801S ; RRID: AB_2798316 |
| Rabbit anti-COL1A1 | abcam | Cat# ab34710; RRID: AB_731684 |
| Rat anti-LY6A | R&D system | Cat# MAB1226; RRID: AB_2243980 |
| Rat anti-Lgr5 | R&D system | Cat# mab8240; RRID: AB_2753324 |
| Goat anti-DPP4 | R&D system | Cat# AF954-SP ; RRID: AB_355739 |
| Goat anti-DLK1 | R&D system | Cat# AF8277; RRID: AB_2861313 |
| Rabbit anti-WNT2 | proteintech | Cat# 27214-1-ap ; RRID: AB_2880804 |
| Rabbit anti-TRPS1 | ABclonal | Cat# A7743; RRID: AB_2772733 |
| Goat anti-ALPL | R&D system | Cat# AF2910; RRID: AB_664062 |
| Sheep anti-ATGL | R&D system | Cat# AF5365; RRID: AB_2165678 |
| Rabbit anti-CRABP1 | Proteintech | Cat# 12588-1-AP ; RRID: AB_2292271 |
| Rabbit-anti-active β-Catenin | Cell Signaling Technology | Cat# 8814; RRID: AB_11127203 |
| Rabbit anti-β-Catenin | Cell Signaling Technology | Cat# 8480; RRID: AB_11127855 |
| Rabbit anti-GSK3β | Cell Signaling Technology | Cat# 12456; RRID: AB_2636978 |
| Rabbit anti-phospho-GSK3β(Ser9) | Cell Signaling Technology | Cat# 5558; RRID: AB_10013750 |
| Rabbit anti-CREB | Cell Signaling Technology | Cat# 9197; RRID: AB_331277 |
| Rabbit anti-Phospho-CREB(Ser133) | Cell Signaling Technology | Cat# 9198; RRID: AB_2561044 |
| Rabbit anti-AKT | Cell Signaling Technology | Cat# 4691S ; RRID: AB_915783 |
| Rabbit anti-Phospho-AKT(Ser473) | Cell Signaling Technology | Cat# 4060S ; RRID: AB_2315049 |
| Rabbit anti-ERK1/2 | Cell Signaling Technology | Cat# 9102S ; RRID: AB_330744 |
| Rabbit anti-Phospho-ERK1/2(Thr202/Tyr204) | Cell Signaling Technology | Cat# 4370S ; RRID: AB_2315112 |
| Rabbit anti-Phospho-NF-κB p65 (Ser536) | Cell Signaling Technology | Cat# 3033; RRID: AB_331284 |
| Rabbit anti-P38 MAPK | Cell Signaling Technology | Cat# 9212S ; RRID: AB_330713 |
| Rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) | Cell Signaling Technology | Cat# 4631S ; RRID: AB_331765 |
| Mouse anti-β-Actin | Proteintech | Cat# 66009-1-Ig ; RRID: AB_2687938 |
| Goat anti-COLIV | Abcam | Cat# ab769; RRID: AB_92262 |
| Rabbit anti-FABP4 | Proteintech | Cat# 15872-1-AP ; RRID: AB_2102440 |
| Rabbit anti-CRAMP | University of California San Diego | Dr. Richard Gallo |
| Rabbit anti-SMA | Proteintech | Cat# 14395-1-ap ; RRID: AB_2223009 |
| Rabbit anti-NF-κB p65 | Cell Signaling Technology | Cat# 8242; RRID: AB_10859369 |
| Rabbit anti-Phospho-C/EBPβ (Thr235) | Cell Signaling Technology | Cat# 3084; RRID: AB_2260359 |
| IRDye 680RD Donkey anti-Mouse | LICOR | Cat# 926-68072; RRID: AB_10953628 |
| IRDye 680RD Donkey anti-Goat IgG (H+L) | LICOR | Cat# 926-68074; RRID: AB_10956736 |
| IRDye 680RD Donkey anti-Rabbit IgG (H + L) | LICOR | Cat# 926-68073; RRID: AB_10954442 |
| IRDye 800CW Donkey anti-Goat IgG (H + L) | LICOR | Cat# 926-32214; RRID: AB_621846 |
| IRDye 800CW Donkey anti-Mouse | LICOR | Cat# 926-32212; RRID: AB_621847 |
| IRDye 800CW Donkey anti-Rabbit | LICOR | Cat# 926-32213; RRID: AB_621848 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Goat IgG (H+L) | Jackson ImmunoResearch | Cat# 705-545-147; RRID: AB_2336933 |
| Cy3 AffiniPure Donkey Anti-Goat IgG (H+L) | Jackson ImmunoResearch | Cat# 705-165-147; RRID: AB_2307351 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson ImmunoResearch | Cat# 712-545-150; RRID: AB_2340683 |
| Cy3-AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson ImmunoResearch | Cat# 712-165-150; RRID: AB_2340666 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch | Cat# 711-545-152; RRID: AB_2313584 |
| Cy3 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch | Cat# 711-165-152; RRID: AB_2307443 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson ImmunoResearch | Cat# 715-545-150; RRID: AB_2340846 |
| Cy3-AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson ImmunoResearch | Cat# 715-165-150; RRID: AB_2340813 |
| Brilliant Violet 480-conjugated AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson ImmunoResearch | Cat# 712-685-150; RRID: AB_2651112 |
| Brilliant Violet 421-conjugated AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson ImmunoResearch | Cat# 712-675-150; RRID: AB_2651110 |
| DyLight 405 AffiniPure Donkey Anti-Goat IgG (H+L) | Jackson ImmunoResearch | Cat# 705-476-147; RRID: AB_2632563 |
| Alexa Fluor 647 AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch | Cat# 711-606-152; RRID: AB_2340625 |
| Alexa Fluor 647 AffiniPure Donkey Anti-Rat IgG (H+L) | Jackson ImmunoResearch | Cat# 712-606-150; RRID: AB_2340695 |
| Alexa Fluor 647 AffiniPure Donkey Anti-Goat IgG (H+L) | Jackson ImmunoResearch | Cat# 705-606-147; RRID: AB_2340438 |
| Biological samples | ||
| mouse skin samples | Xiamen University Laboratory Animal Center | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Eosin Staining Solution | ZSGB-BIO | ZLI-9613 |
| Hematoxylin Staining Solution | ZSGB-BIO | ZLI-9610 |
| 3-Isobutyl-1-methylxanthine (IBMX) | Sigma | I5879 |
| Indomethacin | Sigma | I8280 |
| Dexamethasone | Sigma | D4902 |
| Insulin | Sigma | 91077C |
| DMEM, high glucose | ThermoFisher Scientific | C11965500BT |
| Glutamax | ThermoFisher Scientific | 35050-061 |
| Collagenase D from Clostridium histolyticum | Roche | 11088882001 |
| Antibiotic-Antimycotic | ThermoFisher Scientific | 15240062 |
| 2x SYBR Green qPCR Master Mix | Bimake | B21202 |
| red blood cell lysis buffer | TianGen | RT122-02 |
| SB431542 | Selleckchem | S1067 |
| HyClone Trypsin Protease | Hyclone | SH30042.01 |
| BD Bacto™ Dehydrated Agar | Fisher Scientific | DF0140-01-0 |
| TRI Reagent/TRIzol | Sigma | T9424-200ML |
| UltraPure™ DNase/RNase-Free Distilled Water | ThermoFisher Scientific | 10977015 |
| RNAlater™ Stabilization Solution | ThermoFisher Scientific | AM7021 |
| DnaseI | Solarbio | D8071-100mg |
| Saponin | Sigma | 47036-50G-F |
| Protease inhibitor | Sigma | 11836153001 |
| Wortamannin | Selleck | S2758 |
| U0126-EtOH | Selleck | S1102 |
| SB202190 (FHPI) | Selleck | S1077 |
| BAY 11-7082 | Selleck | S2913 |
| XAV-939 (NVP-XAV939) | Selleck | S1180 |
| 16% PFA | Alfa Aesar | 43368 |
| ProLong™ Gold Antifade Mountant with DAPI | ThermoFisher Scientific | P36931 |
| Collagenase/Dispase | Sigma | 10269638001 |
| Stabilizing Fixative 3X Concentrate | BDIS | 338036 |
| Hifair®II 1st Strand cDNA Synthesis SuperMix for qPCR | Vazyme | 11123ES60 |
| 2x M-PCR OPTI™ Mix (Dye Plus) | Bimake | B45012 |
| Recombinant mouse TGFβ2 | R&D System | 7346-B2-005 |
| recombinant mouse IL1β | R&D system | 401-ML-025 |
| recombinant mouse WNT3A | R&D system | 1324-WN-002 |
| Critical commercial assays | ||
| RNAExpress Total RNA Kit | NCM Biotech | M050 |
| Masson’s Trichrome Stain Kit | Solarbio | G1340-7 |
| Dead Cell Removal Kit | Miltenyi Biotic | 130-090-101 |
| Oil Red O stain kit | Solarbio | G1262 |
| Mouse Direct PCR Kit | Bimake | B40015 |
| Ailzarin red S (Osteoblast stain) | Sigma | A5533 |
| Intracellular Fixation & Permeabilization Buffer Set | eBioscience | 88-8824-00 |
| Deposited data | ||
| Skin developmental scRNAseq data | This study | GSE189241 |
| scRNAseq data of skin wound tissues | This study | GSE196800 |
| Experimental models: Cell lines | ||
| Primary mouse dermal fibroblasts | This study | N/A |
| Experimental models: Organisms/strains | ||
| B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | The Jackson Laboratory | JAX: 007676 |
| C57BL/6-Tg(Ad/poq-Cre/ERT2)1Soff/J | The Jackson Laboratory | JAX: 025124 |
| C57BL/6- Gsk3aflox/floxGsk3bflox/flox | From Wen-Hsien Liu | Chenfeng Liu et al.(https://doi.org/10.1126/sciadv.abg6262) |
| C57BL/6- Il1r1−/− | From Jiahuai Han | Generated by Xiamen University Laboratory Animal Center, Transgenic Animal Facility Core |
| C57BL/6 | From Xiamen University Laboratory Animial Center | N/A |
| Oligonucleotides | ||
| PCR primers, see Table S3 | This study | N/A |
| Software and algorithms | ||
| GraphPad Prism | GraphPad Software, Inc. | N/A |
| FlowJo V10 | FlowJo LLC | N/A |
| Adobe photoshop | Adobe software | N/A |
| Aperio ImageScope | Leica Biosystems | N/A |
| Rstudio | Rstudio | N/A |