TABLE 1.

Amounts of substrate depleted 5 min after initiation of reactions with the indicated enzymes^a

Substrate	nmol of substrate depleted/0.6 nmol of enzyme:
	αB-356βB-356 ISPBPH	αLB400βLB400 ISPBPH	αB-356βLB400 ISPBPH	αLB400βB-356 ISPBPH
2,2′-Dichlorobiphenyl	<10	50	50	30
3,3′-Dichlorobiphenyl	50	<10	<10	30
4,4′-Dichlorobiphenyl	T	T	T	T
2,5-Dichlorobiphenyl	50	60	30	<10
2,2′,5,5′-Tetrachlorobiphenyl	−	35	40	20

^aBPH dox reactions were carried out as described in Materials and Methods. Substrate (100 nmol) was added to initiate each reaction. Numbers refer to the amounts of substrate consumed 5 min after initiation of the reactions. The values are averages of results from three separate experiments with two different enzyme preparations. The variance was less than 10% in all cases. −, no degradation; T, trace amounts of metabolite produced.