Figure 1.

The effect of ATP neutralization by apyrase on LPS-induced ARDS. Mice were treated with 4U/mL apyrase in both prophylactic (left) and therapeutic (right) regimes. (A, B) The BALF cells differential count was measured by flow cytometry with both treatment methods. (C–F) Concentrations of cytokines IL-1β, IL-6, KC, MIP-2 and TNF-α in BALF were measured by ELISA in both regimes. (G, H) Plasma leakage was evaluated spectrophotometrically 30 minutes after Evans blue dye albumin (20 mg/kg), which was injected into the tail vein. Values are given as mean ± SEM. n = 4 mice in each group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 obtained by comparing each group versus LPS (vehicle/LPS in prophylactic or LPS/vehicle in therapeutic regimes). ns means not significant.