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. 2023 Dec 20;625(7993):195–203. doi: 10.1038/s41586-023-06870-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, ARPP19 and FAM122A sequentially inhibit PP2A:B55 activity during mitosis. A, PP2Aa; C, PP2Ac; P, phosphate. b, PP2A:B55 inhibition by ARPP19 (with or without phosphorylation), FAM122A_Nterm and FAM122A_ID (mean ± s.d.; n = 3 experimental replicates). Results representative of n = 3 independent experiments. Unpaired two-tailed t-test with 95% confidence interval was used to compare ARPP19 with tpARPP19 (P < 0.0001) or tpS62tpS104ARPP19 (P < 0.0001). IC₅₀ values are reported in Extended Data Table 1. c, 2D ¹H,¹⁵N HSQC spectrum of ¹⁵N-labelled ARPP19 with or without PP2A:B55. d, Plot of peak intensity versus ARPP19 protein sequence for spectra in c; grey shading highlights ARPP19 residues with reduced intensities in the presence of PP2A:B55. Secondary structure elements based on NMR CSI data are indicated. Colour scheme as in c. e, Plot of peak intensity versus FAM122A protein sequence for FAM122A_Nterm alone (black) and with PP2A:B55 (green); grey shading highlights FAM122A residues with reduced intensities in the presence of PP2A:B55. Secondary structure elements based on NMR CSI data are indicated. f, 2D ¹H,¹⁵N HSQC spectrum of ¹⁵N-labelled ARPP19 with (pink) and without (black) B55_LL; grey shading highlights ARPP19 residues with reduced intensities in the presence of B55_LL. g, Plot of peak intensity versus ARPP19 protein sequence for spectra in f; grey shading highlights ARPP19 residues with reduced intensities in the presence of B55_LL. h, Plot of peak intensity versus FAM122A_ID protein sequence for FAM122A_ID alone (black) and with B55_LL (blue); grey shading highlights FAM122A_ID residues with reduced intensities in the presence of B55_LL.