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. 2023 Dec 20;625(7993):195–203. doi: 10.1038/s41586-023-06870-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a. IC₅₀ curves for PP2A:B55 inhibition by ARPP19 (variants; unphosphorylated and phosphorylated as well as different constructs) and FAM122A_ID variants. IC₅₀ values are reported in Extended Data Table 1. Data are presented as mean values ± s.d. Results representative of n = 3 independent experiments. Unpaired two-tailed t-test with 95% confidence interval was used to compare: ARPP19 with tpARPP19 (p < 0.0001) or tpS62tpS104ARPP19 (p < 0.0001) or tpARPP19_19-75 (p < 0.0001); tpARPP19 with tpS62tpS104ARPP19 (p = 0.02) or tpARPP19_19-75 (p = 0.0002); FAM122A_ID with FAM122A_ID variants ⁸⁴AA⁸⁵ (p < 0.0001) or ⁸⁸AA⁸⁹ (p < 0.0001) or E91K (p < 0.0001) or E92K (p < 0.0001) or E104A (p < 0.0001) or E106A (p = 0.0013). b. FP binding studies to measure the interaction of ARPP19 (unphosphorylated and phosphorylated) and FAM122A_ID variants with PP2A:B55 and B55_LL (gray box). K_D values are reported in Extended Data Table 2. Data are presented as mean values ± s.d. Results representative of n = 3 independent experiments. Unpaired two-tailed t-test with 95% confidence interval was used to compare: PP2A:B55 interaction with ARPP19 vs tpS62tpS104ARPP19 (p = 0.0006); ARPP19 interaction with PP2A:B55 vs B55_LL (p = 0.0016); ARPP19 S104A interaction with PP2A:B55 vs B55_LL (p = 0.0008); PP2A:B55 interaction with FAM122A_ID vs FAM122A_67-120 (p < 0.0001) or ⁸⁴AA⁸⁵ (p < 0.0001) or ⁸⁸AA⁸⁹ (p < 0.0001) or E91K (p < 0.0001) or E92K (p < 0.0001); FAM122A_ID interaction with PP2A:B55 vs B55_LL (p < 0.0001); FAM122A_67-120 interaction with PP2A:B55 vs B55_LL (p < 0.0004).