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. 2023 Dec 20;625(7993):195–203. doi: 10.1038/s41586-023-06870-3

Extended Data Fig. 4. NMR data supporting the interaction of phosphorylated ARPP19 and FAM122A with PP2A:B55.

Extended Data Fig. 4

a. 2D [1H,15N] HSQC spectrum of 15N-labeled tpARPP19 alone (black) and in complex with PP2A:B55 (green). pS62 labeled for clarity. b. Peak intensity vs ARPP19 protein sequence plot for tpARPP19 alone (black) and when bound to PP2A:B55 (green). Secondary structure elements based on NMR CSI data are indicated. c. 2D [1H,15N] HSQC spectrum of 15N-labeled tpS62tpS104ARPP19 alone (black) and in complex with PP2A:B55 (green). pS62 and pS104 labeled for clarity. d. Peak intensity vs ARPP19 protein sequence plot for tpS62tpS104ARPP19 alone (black) and when bound to PP2A:B55 (green). Secondary structure elements based on NMR CSI data are indicated. e. 2D [1H,15N] HSQC spectrum of 15N-labeled FAM122ANterm alone (black) and in complex with PP2A:B55 (green). f. 2D [1H,15N] HSQC spectrum of 15N-labeled FAM122AID alone (black) and in complex with PP2A:B55 (green). g. Peak intensity vs FAM122AID protein sequence plot for FAM122AID alone (black) and when bound to PP2A:B55 (green). Secondary structure elements based on NMR CSI data are indicated.