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. 2023 Dec 20;14:1272918. doi: 10.3389/fimmu.2023.1272918

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Copyright © 2023 Hioki, Ryan, Thesmar, Lynch, Pobezinsky and Pobezinskaya

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular distribution of ZsG in TILs. (A–C) Quantification of frequency of monocytes (A), CD4 (B) and CD8 (C) T cells with different cellular localization of ZsG (left panels) and representative images (right panels). Modulation-morphology feature (blue) and homogeneity feature (red) determine granular versus homogeneous distribution. All images were acquired at 60X magnification. Image display gains for ZsGreen were set to the same parameters with the IDEAS software to allow for visual comparison of intensity between images. Control, cells isolated from B16 tumors; BF, bright field.