Table 1.

Phenotypic identification of immune cells in PBMC, spleen and MLN from 33-day-old pigs fed milk replacer with or without BMOS, HMO or a combination of both.

	CON (n=12)	HMO (n=12)	BMOS (n=12)	BMOS+HMO (n=10)	Statistics
PBMC	Percent of Cells*
T-helper cells	49.4 ± 2.1 a	53.7 ± 2.5 a	49.9 ± 2.0 a	42.7 ± 1.7 b	BMOS*HMO: p=0.007
Cytotoxic T-cells	11.9 ± 1.2	12.0 ± 1.1	12.1 ± 1.1	11.9 ± 1.5	N.S.
Double positive T-cells	5.3 ± 0.6	6.2 ± 0.6	5.7 ± 0.9	5.0 ± 0.5	N.S.
Natural Killer cells	12.5 ± 2.5	10.1 ± 1.9	11.0 ± 2.5	11.5 ± 3.0	N.S.
B-cells	14.0 ± 5.6	12.3 ± 2.3	12.2 ± 2.7	11.9 ± 2.4	N.S.
MLN
T-helper cells	72.6 ± 1.1	72.5 ± 1.0	74.9 ± 1.6	73.6 ± 2.6	N.S.
Cytotoxic T-cells	11.4 ± 0.8	12.8 ± 0.9	11.5 ± 0.9	11.0 ± 1.7	N.S.
Double positive T-cells	8.2 ± 1.1	7.3 ± 0.9	7.3 ± 0.8	8.7 ± 1.0	N.S.
Natural Killer cells	1.4 ± 0.2	1.6 ± 0.1	1.4 ± 0.1	1.4 ± 0.1	N.S.
B-cells	53 ± 3.7	61 ± 5.4	54 ± 5.2	57 ± 5.1	N.S.
Spleen
T-helper cells	46.9 ± 2.5	47.7 ± 3.3	47.4 ± 1.3	41.9 ± 3.1	N.S.
Cytotoxic T-cells	9.9 ± 1.1	10.1 ± 1.1	10.7 ± 1.4	11.7 ± 1.3	N.S.
Double positive T-cells	3.4 ± 0.3	4.1 ± 0.6	4.6 ± 0.8	4.0 ± 0.5	N.S.
Natural Killer cells	5.6 ± 1.5	4.8 ± 1.2	4.6 ± 0.8	4.2 ± 0.7	N.S.
B-cells	41.4 ± 5.0	42.0 ± 4.5	44.4 ± 4.7	40.8 ± 7.7	N.S.

Immune cell population were characterized as: B-cells, MHCII⁺CD21⁺; Natural Killer cells, CD3^-CD4⁺CD8; T-helper cells, CD3⁺CD4⁺CD8⁺, Cytotoxic T-cells, CD3⁺CD4^-CD8⁺; Double positive T-cells, CD3⁺CD4⁺CD8.

*T-cell numbers are presented as % of CD3+ cells; B-cell numbers represent % of total cell count.

Data are presented as mean % ± SEM.

Different letters represent statistical differences at p<0.05; N.S. Not statistically significant.

MLN, mesenteric lymph nodes; PBMC, peripheral blood mononuclear cells; UNST, unstimulated.