Table 1.
Immortalized swine respiratory cells are permissible to hu and swIAV strains of H1N1, H1N2, and H3N2 subtypes.
| Virus name | Abbreviation | Subtype | Origin | Virus Titer 24 hpi | Virus Titer 48 hpi | Virus Titer 72 hpi | Area under curve |
|---|---|---|---|---|---|---|---|
| (log10 TCID50/mL) | (log10 TCID50/mL) | (log10 TCID50/mL) | |||||
| A/Puerto Rico/8/1934 | A/PR8/1935 | H1N1 | Human | 4.88 ± 0.22 | 6.21 ± 0.29 | 5.22 ± 0.11 | 329 ± 11.09 |
| A/New Caledonia/20/99 | A/NewCal/1999 | H1N1 | Human | 4.77 ± 0.27 | 6.11 ± 0.11 | 5.55 ± 0.22 | 327.9 ± 9.90 |
| A/Brisbane/10/2007 | A/Bris/2007 | H3N2 | Human | 3.83 ± 0.16 | 5.22 ± 0.11 | 4.61 ± 0.05 | 272.6 ± 5.97 |
| A/Perth/16/2009 | A/Perth/2009 | H1N1 | Human | 2.38 ± 0.39 | 3.89 ± 0.11 | 2.78 ± 0.27 | 183.9 ± 13.27 |
| A/CA/07/2009 | A/CA/2009 | H1N1 | Human/pdm | 4.77 ± 0.11 | 6.05 ± 0.27 | 6.5 ± 0.5 | 337.8 ± 13.61 |
| A/Victoria/361/2011 | A/VIC/2011 | H3N2 | Human | 4.55 ± 0.05 | 5.33 ± 0.16 | 3.77 ± 0.39 | 282.6 ± 9.77 |
| A/TX/50/2012 | A/TX/2012 | H3N2 | Human | 3.6 ± 0.05 | 4.6 ± 0.05 | 1.17 ± 0.16 | 211.1 ± 4.11 |
| A/swine/MN/A01125993/2012 | A/swine/MN/2012 | H3N2 | Swine | 7.34 ± 0.05 | 7.44 ± 0.11 | 6.89 ± 0.11 | 438.5 ± 4.33 |
| A/Hong Kong/4081/2014 | A/HK/2014 | H3N2 | Human | 3.11 ± 0.11 | 3.88 ± 0.22 | 1.67 ± 0.16 | 181.8 ± 8.09 |
| A/swine/NC/KH1552516/2016 | A/swine/NC/2016 | H3N2 | Swine | 3.55 ± 0.46 | 6.28 ± 0.14 | 7.05 ± 0.24 | 320.5 ± 15.74 |
| A/WI/588/2019 | A/WI/2019 | H1N1 | Human/pdm | 4.6 ± 0.05 | 6.05 ± 0.27 | 5.94 ± 0.24 | 327.2 ± 9.68 |
| A/swine/GA/27480/2019 | A/swine/GA/2019 | H1N2 | Swine | 5.55 ± 0.11 | 7.55 ± 0.22 | 7.83 ± 0.16 | 408.5 ± 8.09 |
Replication kinetics of H1N1, H1N2, and H3N2 hu and swIAV subtypes in immortalized swine respiratory cells. Immortalized swine respiratory cells were seeded onto rat-tail collagen coated 6-well and inoculated independently with H1N1 or H3N2 huIAVs, or H1N2, or H3N2 swIAV strains at an MOI of 0.1. Supernatant was harvested at 0-, 24-, 48-, and 72 h post infection and IAV titers were measured by TCID50. The averages of three replicates. ± represents standard error (n = 3).