TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference |
|---|---|---|
| N43 | ara-14 Δlac-85 acrA1 supE44 galK2 rpsL197 malA1 xyl-5 mtc-1 | 17 |
| LM1237 | F+asnB32 thi-1 relA1 spoT1 | 10 |
| LM2800 | LM1237, but ΔatpIBEFHAGDC750 | 10 |
| LM3115 | LM1237, but ΔatpIBEFHAGDC750 lacUV5 lacY | 11 |
| LM3305 | Spontaneous Suc+ mutant of LM2800 | This study |
| LM3559 | LM2800, but yhiD::mini-Tn5 | This study |
| BOE270 | MC1000, but hsdR highly competent for Ca2+-competent cells | This study |
| MC1000 | araD139 Δ(ara-leu)7697 galU galK Δ(lacIPOZY)X74 rpsL thi-1 | 4 |
| pOMC3694 | pBR322, carrying a mini-Tn5 transposon plus flanking DNA cloned from LM3559 chromosomal DNA | This study |
| pFH2106 | Expression vector derived from pBR322, carrying the lacUV5 promoter, the lacI gene, and a multiple cloning site | F. G. Hansen, personal communication |
| pLAC-yhiD | pFH2106, carrying the yhiD gene from E. coli | This study |
| pLAC-yhiF | pFH2106, carrying the yhiF gene from E. coli | This study |
| pLAC-dctA | pFH2106, carrying the dctA gene from pKAT204 | This study |
| pKAT204 | pBR322, carrying the dctA gene from E. coli | 1 |