TABLE 2.
Growth of an atp deletion mutant, a Suc+ mutant, and the wild-type strain of E. coli on solid medium and various carbon and energy sources
| Strain | Relevant genotype or phenotype | Colony diama (mm) on:
|
||||||
|---|---|---|---|---|---|---|---|---|
| Glycolytic substratesc
|
TCA substratesc
|
|||||||
| Glucose | Pyruvate | Lactate | Acetate | Succinate | Fumarate | Malate | ||
| LM1237 | atp+Suc+ | 1 | 1.5 | 0.7 | 0.7 | 0.7 | 0.4 | 0.8 |
| LM2800 | atp Suc− | 1 | 1 | 0.5 | —b | — | — | — |
| LM3305 | atp Suc+ | 1 | 1 | 0.5 | — | 0.1 | 0.2 | 0.3 |
a
Diameter of single colonies, measured with a microscope and scored after 4 days of incubation at 37°C. The standard error on the determination of colony sizes is less than 15%.
b
—, colony diameter smaller than 0.03 mm.
c
Carbon sources: pyruvate, lactate, acetate, succinate, fumarate, and malate (5 g/liter) and glucose (2 g/liter).