TABLE 3.
Strain | Plasmid | Colony diama (mm)
|
Carbon sourcec | |
---|---|---|---|---|
−IPTG | +IPTGb | |||
LM3305 | pFH2106 | 0.12 | 0.12 | Malate |
LM3305 | pLAC-yhiF | 0.12 | 0.03 | Malate |
LM3305 | pFH2106 | 0.06 | 0.06 | Succinate |
LM3305 | pLAC-yhiF | 0.06 | 0.01 | Succinate |
Diameter of single colonies, measured with a microscope and scored after 4 days of incubation at 37°C. The standard error on the determination of colony sizes is less than 15%.
100 μM IPTG.
Carbon sources at 15 mM.