Table 1.
List of reagents and antibodies used
| Reagent type | Designation | Source | Identifiers | Additional information | Use |
|---|---|---|---|---|---|
| Antibody | Anti-IBA-1(rabbit monoclonal) | Cell signaling | Cat. No. #17198 | IF (1:100) | Marker of microglia/macrophages |
| Antibody |
Anti-rabbit IgG AlexaFluor 546 (rabbit polyclonal) |
Thermo fisher scientific | Cat. No. #A-10040 | IF (1:400) | Secondary antibody for immunofluorescence analyses |
| Antibody |
Anti-rabbit IgG AlexaFluor 488 (rabbit polyclonal) |
Thermo fisher scientific | Cat.No. #A-21206 | IF (1:400) | Secondary antibody for immunofluorescence analyses |
| Antibody |
Anti-mouse IgG AlexaFluor 546 (mouse polyclonal) |
Thermo fisher scientific | Cat.No. #A10036 | IF (1:400) | Secondary antibody for immunofluorescence analyses |
| Nuclear Counterstains | DAPI | Thermo fisher scientific | Cat.No. D1306 | IF (0.5 mg/mL) | Marker of cell nuclei |
| Antibody | Anti-GFAP (mouse monoclonal) | Cell signaling | Cat. No. #3670 |
IF (1:300) WB (1:1000) |
Marker of glial fibrillary acidic protein (glial cells and astrocytes) |
| Antibody | Anti-COX-2 (rabbit monoclonal) | Cell signaling | Cat. No. #12282 |
IF (1:500) WB (1:1000) |
Marker of cyclooxygenase2 protein (inflammatory marker) |
| Antibody | Anti-CtBP2 (mouse) | BD biosciences | Cat. No. 612044 | IF (1:1000) | Marker of C-terminal-binding protein 2 (pre-synaptic contact) |
| Antibody | Anti-NF200 (rabbit polyclonal) | Sigma-Aldrich | Cat. No. N4142 | IF (1:80) | Marker of neurofilaments (afferent fibers) |
| Antibody | Anti-CD68 (mouse monoclonal) | Abcam | Cat. No. ab955 |
WB (1:1000) IF (1:100) |
Phagocytic markers involved in microglia and macrophages activation |
| Antibody | Anti-cleaved-caspase 3 (rabbit polyclonal) | Millipore | Cat. No. #AB3623 | WB (1:100) | Marker of cleaved-caspase-3 protein (active form in apoptotic pathway) |
| Antibody | Anti-Caspase-3 (rabbit polyclonal) | Santa cruz biotechnology | Cat. No. sc-7272 | WB (1:1000) | Marker of total caspase-3 protein |
| Antibody | Anti-NF-κB (rabbit monoclonal) | Cell signaling | Cat. No. #8242 | WB (1:1000) | Transcription factors of the nuclear factor κB (NF-κB) (inflammatory marker) |
| Antibody | Anti-TNF-α (mouse monoclonal) | Santa cruz biotechnology | Cat. No. sc-52746 | WB (1:1000) | Tumor Necrosis Factor α (inflammatory marker) |
| Antibody | Anti-IL-1β (rabbit polyclonal) | Santa cruz biotechnology | Cat. No. sc-7884 | WB (1:1000) | Interleukin-1 β (IL-1β) (inflammatory marker) |
| Antibody | Anti-CXCR1 (rabbit polyclonal) | Thermofisher | Cat. No. #PA5-95749 | WB (1:1000) | Marker of chemokine receptors protein |
| Antibody | Anti-iNOS (rabbit polyclonal) | Thermofisher | Cat. No. # PA1-036 | WB (1:1000) | Marker of Inducible Nitric Oxide Synthase (iNOS) |
| Antibody | Anti-Cx43 (rabbit polyclonal) | Abcam | Cat. No. ab62252 | WB (1:8000) | Marker of Connexin-43 |
| Antibody | Anti-Cx26 (mouse monoclonal) | Thermofisher | Cat. No. # 13-8100 | WB (1 µg/ml) | Marker of Connexin-26 |
| Antibody | Anti-Cx30 (rabbit polyclonal) | Thermofisher | Cat. No. #71-2200 | WB (1 µg/ml) | Marker of Connexin 30 |
| Antibody | Anti-Panx1 (rabbit polyclonal) | Thermofisher | Cat. No. #487900 | WB (1 mg/ml) | Marker of Pannexin 1 |
| Antibody | Anti-Nitro tyrosine (rabbit) | Cell signaling | Cat. No. #06-284 | WB (1:1000) | Marker of protein tyrosine nitration |
| Antibody | Anti-4HNE (rabbit polyclonal) | Alpha diagnostic international | Cat. No. #HNE11-S | WB (1:1000) | Detect the 4-Hydroxy-2-nonenal (HNE) a marker of lipid peroxidation |
| Antibody | Anti-GAPDH (mouse monoclonal) | Abcam | Cat. No. ab8245 | WB (1:10,000) | Loading control |
| Antibody |
Anti-β-tubulin (mouse monoclonal) |
Cell signaling | Cat. No. #2146 | WB (1:5000) | Loading control |
| Antibody |
Anti-phospho-AMPA Receptor 1 (GluA1, Ser845) (rabbit polyclonal) |
Cell signaling | Cat. No. #8084 | WB (1:1000) | Receptor function, trafficking, and conductance |
| Antibody | Anti-AMPA Receptor 1 (GluA1) (rabbit polyclonal) | Cell signaling | Cat. No. #13185 | WB (1:1000) | Total GluA1 levels |