Fig. 1.
Age-dependent gene expression in PCLS under control conditions and upon P. aeruginosa infection. PCLS of young and old mice were infected with 1 × 105 CFU/well PAO1 or D61 or cultured without bacteria (Ctrl), washed 1 h p.i. and transferred into new wells. Infection was continued for 7 h and genome-wide transcriptome analysis was performed on tissue slices of old vs. young mice. (A) Overview of gene expression analysis depicted as unsupervised hierarchical clustering. (B) Venn diagram showing the distribution of differentially expressed genes (≥ 2-fold change, p < 0.05) of lung tissue of old compared to young mice