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. 2024 Jan 4;25:15. doi: 10.1186/s12931-023-02609-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Neutrophil surface markers are age-dependently regulated upon P. aeruginosa exposure. Neutrophils of young and old mice were left unstimulated (Ctrl) or stimulated with bacteria (PAO1 or D61) at MOI of 5 for 4 h. A Receptor expression of CD11b, CD16, CD32, and CD88 was analyzed using flow cytometry and is depicted as mean fluorescence intensity (MFI). B Secretion of TNF-α and IL-1β was analyzed in culture supernatants. n = 6 mice per age group. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with respective control within one age group. # p < 0.05, ## p < 0.01, between the two age groups. $ p < 0.05, $$$ p < 0.001, between two bacterial strains within one age group. All other conditions are not significant