FIG. 2.

Regulation of DAS1 expression in C. boidinii. (A) Western analysis. Protein (20 μg) was separated by SDS-PAGE and detected with anti-DHAS polyclonal antibodies. (B) Northern analysis. Total RNA (20 μg) was loaded in each lane and probed with either the ³²P-labeled DAS1 or the ³²P-labeled ACT1 (actin) DNA as described in Materials and Methods. Carbon sources in each lane are as follows: lanes 1, 8, and 9, glucose; lanes 2, 6, and 7, glycerol; lane 3, methanol; lane 4, oleate; lane 5, d-alanine; and lane 10, glucose plus methanol. Nitrogen sources in each lane are as follows: lanes 1, 2, 3, 4, 5, and 10, NH₄Cl; lanes 6 and 8, methylamine; and lanes 7 and 9, choline. The concentrations of carbon and nitrogen sources are described in Materials and Methods.