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. 1998 Nov;180(22):5885–5890. doi: 10.1128/jb.180.22.5885-5890.1998

TABLE 1.

Relative activities of enzymes related to methanol metabolism during growth on various carbon and nitrogen sourcesa

N source/C source Relative activity (%)
DHAS AOD FDH FLD
NH4Cl/methanol 100 100 100 92
NH4Cl/glucose  NDb ND ND ND
NH4Cl/glycerol ND 20 3.3 22
NH4Cl/oleate ND ND 4.4 4.5
NH4Cl/d-alanine ND ND 3.3 25
NH4Cl/glucose + methanol ND ND ND ND
NH4Cl/glycerol + methanol 67 90 32 67
MAc/glycerol 40 32 36 80
Chld/glycerol 42 63 49 100
MA/glucose ND ND 31 89
Chl/glucose ND ND 78 75
a

Cells were grown and disrupted as described in Materials and Methods. Specific activities of DHAS, AOD, FLD, and FDH for methanol medium are 0.22 ± 0.06, 2.3 ± 0.18, 0.82 ± 0.10, and 0.55 ± 0.08 U/mg of protein, respectively. 

b

ND, not detected. 

c

MA, methylamine. 

d

Chl, choline.