TABLE 1.
N source/C source | Relative activity (%)
|
|||
---|---|---|---|---|
DHAS | AOD | FDH | FLD | |
NH4Cl/methanol | 100 | 100 | 100 | 92 |
NH4Cl/glucose | NDb | ND | ND | ND |
NH4Cl/glycerol | ND | 20 | 3.3 | 22 |
NH4Cl/oleate | ND | ND | 4.4 | 4.5 |
NH4Cl/d-alanine | ND | ND | 3.3 | 25 |
NH4Cl/glucose + methanol | ND | ND | ND | ND |
NH4Cl/glycerol + methanol | 67 | 90 | 32 | 67 |
MAc/glycerol | 40 | 32 | 36 | 80 |
Chld/glycerol | 42 | 63 | 49 | 100 |
MA/glucose | ND | ND | 31 | 89 |
Chl/glucose | ND | ND | 78 | 75 |
Cells were grown and disrupted as described in Materials and Methods. Specific activities of DHAS, AOD, FLD, and FDH for methanol medium are 0.22 ± 0.06, 2.3 ± 0.18, 0.82 ± 0.10, and 0.55 ± 0.08 U/mg of protein, respectively.
ND, not detected.
MA, methylamine.
Chl, choline.