TABLE 2.
Size test for insertion by homologous recombination
| Vector or strain | Size difference (kbp)a | Sizes (kbp) of new fragments in the Kmr strainsb |
|---|---|---|
| Vector | 4.2 | NA |
| Mxa191 | 3.6 | 6.7, 1.3 |
| Mxa210 | 3.8 | 4.5, 3.6 |
| Mxa213 | 4.0 | 4.5, 1.4 |
| Mxa259 | 4.4 | 5.3, 1.1 |
| Mxa264 | 3.9 | 4.7, 1.1 |
| Mxa287 | 4.2 | 4.3, 1.5 |
| Mxa296 | 4.1 | 5.0, 3.8 |
a
Difference = (sum of the sizes of the two new fragments in mutant DNA) − size of the fragment in wild-type DNA. Data were taken from the Southern blots shown in Fig. 3A.
b
The first-listed size in each entry is the size of the band which hybridizes to pBGS18. NA, not applicable.