Figure 4.

FcγR binding with hF5-WT and hF5-LALA. hF5-WT and hF5-LALA mutant Ab binding murine FcγRs I (a), IIb (b), III (c), and IV (d). hF5-WT and hF5-LALA Ab binding to human FcγRI (e). hF5-WT Ab binds to both mouse and human FcγR I orthologs, and mouse FcγRIV, but fails to bind to murine FcγR IIb and III, while the hF5-LALA mutant exhibits abrogated binding to all murine FcγRs and substantially reduced binding to human FcγRI. Data are from experimental conditions performed in triplicate, and the error bars represent the standard deviation from the mean. P values considered significant if < .05 (*), or very significant if < 0.01 (**), <.001 (***), or < .0001 (****).